Imaging Facility
The shared Boston College imaging facility has 712 sq. ft. of space within Higgins Hall, 物理和生物大楼于2002年在BC大学主校区完工. 该设施有五个独立的显微镜空间和设施主任的办公空间(Bret Judson),其中还设有几个工作站,供设施用户进行图像分析. 工作台空间可用于设置实验,设施内有通风柜.
Acknowledgement of Core Facility Document
![Axioplan 2](/content/bc-web/research/sites/vice-provost-for-research/about/research-facilities/imaging-facility/_jcr_content/par/bc_image_content/image.img.jpg/1627503548992.jpg)
Axioplan 2. Zeiss Axioplan 2Axioplan 2成像是一种通用的直立显微镜,用于光(透射和入射)和荧光显微镜. 荧光照明由Lumencor SOLA光引擎提供. 这种光引擎通过消除对汞灯的需求而更加人性化和环保. 显微镜由Micromanager软件控制(操作冷却ccd黑白Hamamatsu相机),并允许在DIC和荧光通道(DAPI)中收集延时图像, FITC, TRITC). 一个客观加热器确保组织培养细胞的完整性在延时实验. In addition, the microscope is equipped with a digital color camera (SPOT, Insight)能够收集染色的组织学组织切片.
![Axioimager Z2](/content/bc-web/research/sites/vice-provost-for-research/about/research-facilities/imaging-facility/_jcr_content/par/bc_image_content_0/image.img.jpg/1655827996958.jpg)
Axioimager Z2. 这是一台直立显微镜,配备了两种传输(包括DIC), and phase) and fluorescence imaging. 荧光照明由EXFO LED光源提供,消除了汞灯. 它是全自动的,允许在多个X, Y和Z位置进行采集. The microscope is equipped with objectives from 5x to 100x. Image capture and analysis is through the Zeiss ZEN software. 对于大型数据集,有一个运行ZEN的离线图像分析工作站.
![confocal](/content/bc-web/research/sites/vice-provost-for-research/about/research-facilities/imaging-facility/_jcr_content/par/bc_image_content_1/image.img.jpg/1655828004261.jpg)
Confocal SP5. Leica TCS SP5 scanning confocal microscope: 该系统是理想的3D成像的紫外和可见荧光团在固定或活样品. 对于活样品的长期成像,该系统设置有Oko-lab环境孵育室,允许温度从环境温度到40°C. 对于需要微流体能力的电子游戏正规平台人员,该系统可以与CellASIC Onix微灌注系统一起运行. 该系统可以将透射光技术与共聚焦成像技术相结合,形成一个高度灵活的平台. 运行该系统的徕卡软件允许时间推移,Z堆栈和3D重建.
![Zeiss LSM 880-AS FAST Elyra.S1](/content/bc-web/research/sites/vice-provost-for-research/about/research-facilities/imaging-facility/_jcr_content/par/bc_image_content_2/image.img.jpg/1655828012420.jpg)
Zeiss LSM 880-AS FAST Elyra.S1: 该系统将多种技术结合到一个灵活的平台中. 该系统是蔡司最新的共聚焦显微镜,能够在405nm至633nm的多个波长下对样品进行成像,放大倍数为10倍至100倍. 该系统包括两种“超分辨率”模式——airscan /FAST单元和Elyra单元. airscan技术将横向分辨率提高到140纳米,轴向分辨率提高到400纳米. 此外,Airyscan模块可以在“FAST”模式下运行实时样品. 对于需要比airscan提供更高分辨率的用户,系统具有Elyra单元,可以提供120nm的横向分辨率和300nm的轴向分辨率. 根据实验需要,用户可以在这些技术之间自由切换. 对于需要温度控制的样品,系统配备了加热和冷却插件.
![Evos](/content/bc-web/research/sites/vice-provost-for-research/about/research-facilities/imaging-facility/_jcr_content/par/bc_image_content_3/image.img.jpg/1655828050621.jpg)
Evos. Life Technologies EVOS FL: 这是一个倒置显微镜,允许在各种配置下成像,从载玻片到96孔板. 显微镜配备了各种干物镜,可以很容易地升级,包括油浸物镜. 照明全部是LED,消除了传统上用于荧光照明的汞灯.
![Spinning Disk.](/content/bc-web/research/sites/vice-provost-for-research/about/research-facilities/imaging-facility/_jcr_content/par/bc_image_content_4/image.img.jpg/1663883297523.jpg)
Spinning Disk. 3i (Intelligent Imaging Innovations) spinning disk confocal: 该设施将智能成像创新(3I)集成在蔡司Axio Observer全自动倒置显微镜上的横河旋转盘共聚焦. The spinning disk system includes: 4 laser lines, a Prime 95B sCMOS camera (Photometrics), temperature controlled stage adapter (Okolab), Vector2系统(3I)用于光动力学实验,Ablate模块(3I)用于细胞和组织的靶向损伤.
![Zeiss Axio Zoom V16](/content/bc-web/research/sites/vice-provost-for-research/about/research-facilities/imaging-facility/_jcr_content/par/bc_image_content_4_c/image.img.jpg/1655827940611.jpg)
Zeiss Axio Zoom V16. This is a stereo microscope with a 16x zoom range. 它配备了用于透射/反射光成像的彩色相机和用于荧光成像的单色相机,带有4个荧光立方体(DAPI), GFP, RFP and CY5). Additionally, the microscope is equipped with the Apotome.3 for optical sectioning when using fluorescence. 它是完全机动的,允许获得一个大的Z范围. Image capture and analysis is through the Zeiss ZEN software. 这种显微镜非常适合于高分辨率的大标本成像. 对于大型数据集,有一个运行ZEN的离线图像分析工作站.
Workstation. Workstation with image analysis software:有一台离线图像分析计算机,可同时运行Windows和Mac操作系统. 这台电脑可以用于任何离线图像分析,包括徕卡LAS, Fiji, and Prism software.
The microscopes are located in room 525. For all microscope questions and training, please contact: Bret Judson.
Facility Documentation
Acknowledgement of Core Facility Document
请注意,在BC核心设施和充电中心进行的所有工作都应该得到适当的认可. 如果您正在发布或展示在BC核心设施和充电中心获得的数据, 请在您的手稿/海报/报告的致谢部分包括以下声明, "The authors would like to thank the Boston College
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